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Aug 19, 2003· From the fungus Aspergillus niger, we identified a new gene encoding protein EstA, a member of the alpha/beta-hydrolase fold superfamily but of unknown substrate specificity. EstA was overexpressed and its crystal structure was solved by molecular replacement using a lipase-acetylcholinesterase chimera template.
Jun 13, 2019· Single cell protein production by a local Aspergillus niger in solid state fermentation using rice straw pulp as carbon source: effects of fermentation variables. S D Said 1, M Zaki 1, T M Asnawi 1 and E Novita 2. Published under licence by IOP Publishing Ltd IOP Conference Series: Materials Science and Engineering, Volume 543, Number 1
Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose.
Mar 21, 2021· Aspergillus niger anip7-gfp2 (formerly denoted as A. niger ARAn701) was kindly provided by Prof. Spieß and Mathias Papenfuß at the Technische Universität Braunschweig (Germany). The strain is deviated from the protease deficient A. niger AB1.13 23 and features the recombinant expression of GFP under control of the glaA promotor.
Oct 08, 2012· Production of Single Cell Protein in Stickwater by Lactobacillus acidophilus and Aspergillus niger. Journal of Aquatic Food Product Technology, 2012. A. Abedian. ... Production of Single Cell Protein in Stickwater by Lactobacillus acidophilus and Aspergillus niger. A. Abedian. Related Papers. Co-culture a great promising method in single cell ...
An Aspergillus niger (A. niger) strain was identified that produces an extracellular proline-specific protease with an acidic pH optimum. On the basis of sequence similarities, we conclude that the A. niger-derived enzyme probably belongs to the S28 family of clan SC of serine proteases rather than the S9 family to which prolyl oligopeptidases ...
Dec 28, 2010· Rho GTPases are signalling molecules regulating morphology and multiple cellular functions including metabolism and vesicular trafficking. To understand the connection between polarized growth and secretion in the industrial model organism Aspergillus niger, we investigated the function of all Rho family members in this organism.We identified six Rho GTPases in its genome and used loss …
The Aspergillus niger RmsA protein. March 2010; ... Polarization of signaling molecules is also fundamental for the lifestyle of filamentous fungi such as Aspergillus niger and essential for their ...
Jan 28, 2007· The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly …
Jan 10, 2002· The fungus Aspergillus niger is a main source of industrial cellulase. beta-1,4-Endoglucanase is the major component of cellulase from A. niger. In spite of widespread applications, little is known about the structure of this enzyme. Here, the structure of beta-1,4-endoglucanase from A. niger (EglA) was determined at 2.1 A resolution.
splice isoforms or post-translational modifications are collapsed, i.e. each node represents all the proteins produced by a single, protein-coding gene locus.
Dec 17, 2020· Improvementof Foreign-Protein Production in Aspergillus niger var.awamori by Constitutive Induction of the Unfolded-ProteinResponse Authors : Mari Valkonen [email protected], Michael Ward, Huaming Wang, Merja Penttilä, and Markku Saloheimo
Apr 06, 2016· Extracellular protein analysis during solid state fermentation with Aspergillus niger The secretome of A. niger CICC 41258 in FRSM was separated using 2D-DIGE ( Fig 5B ). The analysis of the negative control (raw RSM) showed that there were fewer protein spots from unfermented RSM in the 2-DE maps ( Fig 5A ) than that from fermented RSM.
Single-cell protein (SCP) and cellulase production by Aspergillus niger AS-101, grown on alkalitreated corn cobs, was studied under various cultural conditions. The maximum yields of SCP and cellulase, under solid-state fermentation were obtained when the culture was incubated at pH 5.5 for 18 d at 30°C with 12% substrate.
May 12, 2003· Aims: To increase the expression level of heterologous gene in Aspergillus niger. Methods and Results: Inserting multiple copies of an activator protein-binding site originally encoded in the cis-regulatory region of A. niger glucoamylase gene (glaA), CCAAT-containing sequence, into the promoter of an expression plasmid greatly enhanced the production of heterologous protein.
Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain : 0.580: An01g11120: Multicopper oxidase family protein; Function: the S. cerevisiae Fet5 protein is a iron transport multicopper oxidase ... A. niger, ATCC 16888, Aspergillus niger Tiegh., CBS 554 ...
Aspergillus niger is a haploid filamentous fungus which is used for waste management and biotransformations in addition to its industrial uses, such as production of citric acid and extracellular enzymes. It is most commonly found in decaying vegetation, soil, or plants, but it cannot be considered particularly dangerous in comparison to Aspergillus fumigatus, which is the most prevalent ...
May 08, 2015· The Penicillium chrysogenum antifungal protein PAF is toxic against potentially pathogenic Ascomycetes. We used the highly sensitive aequorin-expressing model Aspergillus niger to identify a defined change in cytoplasmic free Ca 2+ dynamics in response to PAF. This Ca 2+ signature depended on an intact positively charged lysine-rich PAF motif. By combining Ca 2+ measurements in …
Feb 01, 2001· The effect of various treatments that block protein secretion was visualized in Aspergillus niger using a strain expressing a glucoamylase–GFP fusion …
An efficient cellulolytic mold, Aspergillus niger AS-101 was used to produce single cell protein from alkalitreated corn cobs. Microbial biomass obtained after 6 days of fermentation was evaluated for its various quality components. Single cell protein product contained 30.4% crude protein and had a …
Oct 07, 2021· Improved recombinant protein production in Aspergillus oryzae lacking both α-1,3-glucan and galactosaminogalactan in batch culture with a lab-scale bioreactor Author links open overlay panel Hikaru Ichikawa 1 ‡ Ken Miyazawa 1 ‡ § Keisuke Komeiji 1 Shunya Susukida 1 Silai Zhang 2 Kiyoaki Muto 1 Ryutaro Orita 1 Ayumu Takeuchi 1 Yuka Kamachi ...
Oct 23, 2020· The filamentous fungus Aspergillus niger is one of the main cell factories used nowadays in the industry for homologous or heterologous protein production due to its extraordinary ability for protein expression and secretion [1,2,3].The Design-Build-Test-Learn (DBTL) cycle is an increasingly adopted systematic metabolic engineering strategy to achieve the desired outcome through …
Protein target information for Glucoamylase (Aspergillus niger). Find diseases associated with this biological target and compounds tested against it in bioassay experiments.
Two inhibitors of the biosynthesis of as pergillin, the black spore pigment of Aspergillus niger, have been investigated and a pathway for aspergillin biosynthesis is suggested based on these results. Two inhibitors of the biosynthesis of aspergillin, the black spore pigment of Aspergillus niger, have been investigated. 2,4-Dithiopyrimidine exerted its inhibitory effect by intracellularly ...
Aspergillus
Aspergillus niger is still less reported by researchers. This research evaluates the possibility to utilize LHW pretreatment rice straw pulp for SCP production by a local Aspergillus niger and the effects of initial pH, initial moisture content, the C/N ratio and fermentation time …
Aspergillus niger anip7-gfp2 (formerly denoted as A. niger ARAn701) was kindly provided by Prof. Spieß and Mathias Papenfuß at the Technische Universität Braunschweig (Germany). The strain is devi-ated from the protease deficient A. niger AB1.1323 and features the recombinant expression of GFP under control of the glaA promotor.
Aspergillus niger resulted in an enormous reduction of extracellular product degrading activity. Degradation of the proteolytic susceptible A. niger PelB protein was reduced 1000-fold in an A. niger production strain, in which three major proteases were disrupted. Also intracellular proteases are important for protein secretion.
Jun 28, 2017· Even though cleavage at Pro was not particularly pronounced, nepenthesin I achieved a superior coverage of the disordered region compared to pepsin for this protein. Here, we characterize the performance of a genuine Pro-specific peptidase from Aspergillus niger the Prolyl endopeptidase (An-PEP) in HDX-MS applications. Currently, due to its Pro ...
The study not only introduces a protocol for preparing ghost cells from Aspergillus niger but also enables the isolation of both of protein and DNA. The idea, the concept and the tools used in this study could establish a more sensitive method for protein and …
Oct 21, 2003· In this work, a recombinant Aspergillus niger strain AB4.1[pgpdAGLAGFP]#11 which carries a glucoamylase (GLA)‐green fluorescent protein (GFP) fusion gene was selected as a model system to study the effects of bioprocess parameters—agitation intensity, initial glucose concentration, initial yeast extract concentration, and dissolved oxygen ...
Feb 19, 2018· Growth Aspergillus niger on solid state fermentation media for produce protease. The best condition for protease production in the moisture is 1:30 and incubation at 40˚C for 8day at PH 8. Inoculation with 2.2×10 4 cell/ml of fungal media containing, different nitrogen sources the peptone the bast. Protease was precipitated from production media by 70% saturation of ammonium sulphat with …
Jan 16, 2018· Sugarcane distillery waste water (SDW) or vinasse is the residual liquid waste generated during sugarcane molasses fermentation and alcohol distillation. Worldwide, this effluent is responsible for serious environmental issues. In Reunion Island, between 100 and 200 thousand tons of SDW are produced each year by the three local distilleries. In this study, the potential of Aspergillus niger to ...